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Mosley, A.* ; Graw, J. ; Delamaere, N.A.*

Altered Na,K-ATPase Pattern in y-Crystallin Mutant Mice.

Invest. Ophthalmol. Vis. Sci. 43, 1517-1519 (2002)
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PURPOSE. Na,K-adenosine triphosphatase (ATPase) activity is elevated in the lenses of murine cataract Cryget and Crygens mutant mice. In the present study, the expression of Na,K-ATPase 1, 2, and 3 catalytic subunit polypeptides was examined in the lenses of these mutant mice. METHODS. Membrane material was isolated from lenses and brain of 3-week-old wild-type mice, as well as heterozygous and homozygous mutant mice. Microsomal membranes were prepared by centrifugation of the homogenized material, and Na,K-ATPase polypeptides were detected by immunoblot analysis with antibodies directed against the Na,K-ATPase isoforms 1, 2, and 3. RESULTS. For the Na,K-ATPase isoforms 2 and 3, membrane material obtained from the homozygous cataract mutants showed dense immunoblot bands that were not detected in material obtained from wild-type mice. An apparent increase of the 1 Na,K-ATPase isoform band density was also detected in lens material from the homozygous mutant mice. The Na,K-ATPase 3 polypeptide was also detected in lens membrane material obtained from heterozygous mice of both mutant strains. The 2 Na,K-ATPase polypeptide was observed in lens membrane material obtained from heterozygous Cryget mice, and a less dense band was detected in heterozygous Crygens mice. Band densities of Na,K-ATPase subunits 1, 2, and 3 detected in brain membrane material were similar in both mutant and wild-type mice. CONCLUSIONS. The immunoblot results suggest that the abundance of Na,K-ATPase polypeptide is increased in the lens of the cataract mouse mutant but is not altered in the brain. The expression of the 2 and 3 isoform proteins of Na,K-ATPase is markedly upregulated in the cataractous lens.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 0146-0404
e-ISSN 1552-5783
Quellenangaben Band: 43, Heft: , Seiten: 1517-1519 Artikelnummer: , Supplement: ,
Verlag Association for Research in Vision and Ophthalmology (ARVO)
Begutachtungsstatus Peer reviewed