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Cloning of phospholipid hydroperoxide glutathione peroxidase (PHGPx) as an anti-apoptotic and growth promoting gene of Burkitt lymphoma cells.

BioFactors 14, 179-190 (2001)
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Burkitt Lymphoma (BL) cells are highly sensitive to suboptimal growth conditions and undergo apoptosis when seeded at low cell density or reduced serum concentration. Irradiated fibroblasts or a mix of pruvate, alpha -thioglycerol, and bathocuproine disulfonate can protect BL cells from apoptosis induced by lowering cell density or serum concentration by promoting cystine uptake in the cells. The availability of cystine is the limiting factor for glutathione biosynthesis in BL cells and thus for the ability of the cells to cope with oxidative stress. We have set up an expression cloning strategy to clone genes that protect BL cells from apoptosis induced by low cell density and/or serum. Using this approach we have cloned among others the cDNA for Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx).
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Publication type Article: Journal article
Document type Scientific Article
Keywords PHGPx; apoptosis; GSH biosynthesis; cystine transport; Burkitt Lymphoma cells; expression cloning
ISSN (print) / ISBN 0951-6433
e-ISSN 1872-8081
Journal BioFactors
Quellenangaben Volume: 14, Issue: , Pages: 179-190 Article Number: , Supplement: ,
Publisher Wiley
Reviewing status Peer reviewed