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Activity of native hydrolytic enzymes and their association with the cell wall of three ectomycorrhizal fungi.

Mycorrhiza 23, 185-197 (2013)
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The ecological and biogeochemical relevance of hydrolytic enzymes associated with the fungal cell wall has been poorly studied in ectomycorrhizal (ECM) fungi. We used a modified sequential extraction procedure to investigate the activity of various hydrolytic enzymes (β-glucosidase, acid-phosphatase, leucine-aminopeptidase, chitinase, xylanase and glucuronidase) and their association with the cell wall of three ECM fungi (Rhizopogon roseolus, Paxillus involutus and Piloderma croceum). Fungi were grown on C-rich solid medium under three different P concentrations (3.7, 0.37 and 0.037 mM). The sequential extraction procedure classifies enzymes as: (a) cytosolic, (b) loosely bound, (c) hydrophobically bound, (d) ionically bound and (e) covalently bound. Results showed that for the same fungus absolute enzymatic activity was affected by P concentration, whilst enzymatic compartmentalization among the cytosol and the cell wall fractions was not. The association of enzymes with the cell wall was fungus- and enzyme-specific. Our data indicate also that enzymes best known for being either extracellular or cytosolic or both, do act in muro as well. The ecological implications of cell wall-bound enzymes and the potential applications and limitations of sequential extractions are further discussed.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cell wall-bound enzymes; Paxillus involutus; Piloderma croceum; Rhizopogon roseolus; Sequential extraction; Multivariate analysis; General linear model; Bound Phosphatase-activity ; Paxillus-involutus ; Candida-albicans ; Phanerochaete-chrysosporium ; Beta-glucosidases ; Acid-phosphatase ; Soil ; Proteins ; Nutrients ; Cloning
ISSN (print) / ISBN 0940-6360
e-ISSN 1432-1890
Zeitschrift Mycorrhiza
Quellenangaben Band: 23, Heft: 3, Seiten: 185-197 Artikelnummer: , Supplement: ,
Verlag Springer
Begutachtungsstatus Peer reviewed