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A physical, genetic and functional sequence assembly of the barley genome.

Nature 491, 711-716 (2012)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Messenger-rna Decay ; Mildew Resistance Locus ; Hordeum-vulgare L. ; Noncoding Rnas ; Evolution ; Map ; Expression ; Rice ; Arabidopsis ; Reveals
Reviewing status