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Eri1 degrades the stem-loop of oligouridylated histone mRNAs to induce replication-dependent decay.

Nat. Struct. Mol. Biol. 20, 73-81 (2013)
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The exoRNase Eri1 inhibits RNA interference and trims the 5.8S rRNA 3' end. It also binds to the stem-loop of histone mRNAs, but the functional importance of this interaction remains elusive. Histone mRNAs are normally degraded at the end of S phase or after pharmacological inhibition of replication. Both processes are impaired in Eri1-deficient mouse cells, which instead accumulate oligouridylated histone mRNAs. Eri1 trims the mature histone mRNAs by two unpaired nucleotides at the 3' end but stalls close to the double-stranded stem. Upon oligouridylation of the histone mRNA, the Lsm1-7 heteroheptamer recognizes the oligo(U) tail and interacts with Eri1, whose catalytic activity is then able to degrade the stem-loop in a stepwise manner. These data demonstrate how degradation of histone mRNAs is initiated when 3' oligouridylation creates a cis element that enables Eri1 to process the double-stranded stem-loop structure.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Binding-protein ; Translation Factors ; Dna-replication ; Microrna ; Yeast ; Degradation ; Uridylation ; Complex ; 3'-end ; 3'-exonuclease
ISSN (print) / ISBN 1545-9993
e-ISSN 1545-9985
Quellenangaben Band: 20, Heft: 1, Seiten: 73-81 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort New York, NY
Begutachtungsstatus Peer reviewed