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Zivraj, K.H.* ; Rehbein, M.* ; Olschläger-Schütt, J.* ; Schob, C.* ; Falley, K.* ; Buck, F.* ; Schweizer, M.* ; Schepis, A.* ; Kremmer, E. ; Richter, D.* ; Kreienkamp, H.J.* ; Kindler, S.*

The RNA-binding protein MARTA2 regulates dendritic targeting of MAP2 mRNAs in rat neurons.

J. Neurochem. 124, 670-684 (2013)
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Dendritic targeting of mRNAs encoding the microtubule-associated protein 2 (MAP2) in neurons involves a cis-acting dendritic targeting element. Two rat brain proteins, MARTA1 and MARTA2, bind to the cis-element with both high affinity and specificity. In this study, affinity-purified MARTA2 was identified as orthologue of human far-upstream element binding protein 3. In neurons, it resides in somatodendritic granules and dendritic spines and associates with MAP2 mRNAs. Expression of a dominant-negative variant of MARTA2 disrupts dendritic targeting of endogenous MAP2 mRNAs, while not noticeably altering the level and subcellular distribution of polyadenylated mRNAs as a whole. Finally, MAP2 transcripts associate with the microtubule based motor KIF5 and inhibition of KIF5 but not cytoplasmic dynein function disrupts extrasomatic trafficking of MAP2 mRNA granules. Thus, in neurons MARTA2 appears to represent a key trans-acting factor involved in KIF5c-mediated dendritic targeting of MAP2 mRNAs.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Microtubule; RNA transport; trans-acting factor; ribonucleoprotein particle; molecular motor; kinesin; Mental-retardation Protein ; Single-stranded-dna ; Ribonucleoprotein Complexes ; Cytoplasmic Localization ; Kh Domains ; Transport ; Translation ; Element ; Ksrp ; Expression
ISSN (print) / ISBN 0022-3042
e-ISSN 1471-4159
Quellenangaben Band: 124, Heft: 5, Seiten: 670-684 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed