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MALDI imaging mass spectrometry for direct tissue analysis.

Methods Mol. Biol. 931, 537-546 (2013)
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
MALDI (Matrix-Assisted Laser Desorption/Ionization) Imaging mass spectrometry is a powerful new method for analyzing the spatial distribution of molecules in tissues. Several different classes of cellular constituents such as proteins, peptides, lipids, and small molecules can be analyzed in situ while maintaining the morphological integrity of the tissue. This allows a correlation of the morphology with the previously acquired molecular patterns. By this, specific molecules can be clearly assigned to their cellular origin. Here, we will present a protocol for the analysis of proteins in tissues which are either native or alcohol-fixed and paraffin-embedded.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Herausgeber Taatjes, D.J.* ; Roth, J.*
Schlagwörter MALDI Imaging; Imaging mass spectrometry; In situ proteomics; Tissue proteomics; Morphology-based tissue analysis
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Konferenztitel Cell Imaging Techniques : Methods and Protocols
Quellenangaben Band: 931, Heft: , Seiten: 537-546 Artikelnummer: , Supplement: ,
Reihe Methods in Molecular Biology
Verlag Springer
Verlagsort Berlin [u.a.]
Begutachtungsstatus Peer reviewed