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Gözdereliler, E. ; Boon, N.* ; Aamand, J.* ; de Roy, K.* ; Granitsiotis, M.S. ; Albrechtsen, H.J.* ; Sørensen, S.R.*

Comparing metabolic functionalities, community structures, and dynamics of herbicide-degrading communities cultivated with different substrate concentrations.

Appl. Environ. Microbiol. 79, 367-375 (2013)
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Two 4-chloro-2-methylphenoxyacetic acid (MCPA)-degrading enrichment cultures selected from an aquifer on low (0.1 mg liter(-1)) or high (25 mg liter(-1)) MCPA concentrations were compared in terms of metabolic activity, community composition, population growth, and single cell physiology. Different community compositions and major shifts in community structure following exposure to different MCPA concentrations were observed using both 16S rRNA gene denaturing gradient gel electrophoresis fingerprinting and pyrosequencing. The communities also differed in their MCPA-mineralizing activities. The enrichments selected on low concentrations mineralized MCPA with shorter lag phases than those selected on high concentrations. Flow cytometry measurements revealed that mineralization led to cell growth. The presence of low-nucleic acid-content bacteria (LNA bacteria) was correlated with mineralization activity in cultures selected on low herbicide concentrations. This suggests that LNA bacteria may play a role in degradation of low herbicide concentrations in aquifers impacted by agriculture. This study shows that subpopulations of herbicide-degrading bacteria that are adapted to different pesticide concentrations can coexist in the same environment and that using a low herbicide concentration enables enrichment of apparently oligotrophic subpopulations.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Nucleic-acid Content ; 16s Ribosomal-rna ; Gulf-of-mexico ; Flow-cytometry ; Bacterial-cells ; Multiphasic Kinetics ; Planktonic Bacteria ; Agricultural Soils ; Gut Microbiota ; Mineralization
ISSN (print) / ISBN 0099-2240
e-ISSN 1098-5336
Quellenangaben Volume: 79, Issue: 1, Pages: 367-375 Article Number: , Supplement: ,
Publisher American Society for Microbiology (ASM)
Reviewing status Peer reviewed