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Oral secretions from herbivorous lepidopteran larvae exhibit ion channel-forming activities.
FEBS Lett. 581, 898-904 (2007)
Feeding insects introduce oral secretions (OS) into the wounded tissue of the attacked plant. Various OS-derived molecules must be involved in subsequent processes including the induction of plant defence reactions. Using the planar lipid bilayer membrane technique, isolated OS were analyzed with respect to their membrane activities. Transmembrane ion fluxes were generated by OS of eight different lepidopteran larvae, all of which form comparable ion channels in artificial membranes. Currents were characterized by long lasting open times and conductivities from 250pS up to 1100pS. Channels formed by Spodoptera exigua secretions showed a preference for cations over anions. OS also induced a transient increase of the cytosolic calcium concentration in soybean cells, determined by the aequorin technique. Known compounds of the OS, fatty-acid-glutamine conjugates, also interfered with the membrane but were unable to form stable channels. Since ion fluxes and depolarization are early responses upon insect feeding, OS-derived components may be involved in the elicitation process by direct interaction with the plant membranes.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Ion fluxes; Linolenoyl–glutamine; Oral secretions; Planar lipid bilayer; Plant–insect interactions
ISSN (print) / ISBN 0014-5793
Journal FEBS Letters
Quellenangaben Volume: 581, Issue: 5, Pages: 898-904
Reviewing status Peer reviewed
Institute(s) Institute of Biochemical Plant Pathology (BIOP)