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Egloff, S.* ; O'Reilly, D.* ; Chapman, R.D. ; Taylor, A.* ; Tanzhaus, K.* ; Pitts, L.* ; Eick, D. ; Murphy, S.*

Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression.

Science 318, 1777-1779 (2007)
Open Access Green as soon as Postprint is submitted to ZB.
RNA polymerase II (Pol II) transcribes genes that encode proteins and noncoding small nuclear RNAs (snRNAs). The carboxyl-terminal repeat domain (CTD) of the largest subunit of mammalian RNA Pol II, comprising tandem repeats of the heptapeptide consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7, is required for expression of both gene types. We show that mutation of serine-7 to alanine causes a specific defect in snRNA gene expression. We also present evidence that phosphorylation of serine-7 facilitates interaction with the snRNA gene-specific Integrator complex. These findings assign a biological function to this amino acid and highlight a gene type-specific requirement for a residue within the CTD heptapeptide, supporting the existence of a CTD code.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 0036-8075
e-ISSN 1095-9203
Journal Science
Quellenangaben Volume: 318, Issue: 5857, Pages: 1777-1779 Article Number: , Supplement: ,
Publisher American Association for the Advancement of Science (AAAS)
Reviewing status Peer reviewed