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Effect of canine surfactant protein (SP-A) on the respiratory burst of phagocytic cells.

FEBS Lett. 270, 90-94 (1990)
Open Access Green as soon as Postprint is submitted to ZB.
Cells obtained from bronchoalveolar lavage, or neutrophils of peripheral blood of dog, were incubated with the canine surfactant-associated protein A (SP-A). A significant decrease of the production of Superoxide anion was observed after subsequent stimulation with phorbol-12-myristate-13-acetate (PMA) as measured by the lucigenin-dependent chemiluminesence (CL). Several other proteins used for control experiments did not decrease lucigenin-dependent CL, indicating a specific effect of SP-A on phagocytes. Treatment of SP-A with collagenase prior to incubation with neutrophils destroyed the depleting effect on oxygen radical production of PMA-stimulated cells. We propose that SP-A acts as a regulatory factor of the respitatory burst of alveolar macrophages and neutrophils in the lungs. The inhibitory effect of SP-A is down-regulated by collagenase released from stimulated alveolar macrophages.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 0014-5793
e-ISSN 1873-3468
Journal FEBS Letters
Quellenangaben Volume: 270, Issue: 1-2, Pages: 90-94 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed