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Identification and in Situ Localization of the Insulin-like Growth Factor-II/mannose-6-phosphate ( IGF-II/M6P) Receptor in the Rat Gastrointestinal Tract: Comparison with the IGF-I Receptor.

Endocrinology 129, 1769-1778 (1991)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
In the present study we investigated pharmacological, biochemical, and immunological characteristics as well as the tissue distribution of the insulin-like growth factor-II/man nose-6-phosphate (IGF-II/M6P) receptor in the rat gastrointestinal tract, and compared the data with those from corresponding experiments for the IGF-I receptor. Competitive binding and affinity cross-linking studies with [125I]IGF-II, and [125I]IGF-I respectively, in rat jejunum yielded results analogous to those previously obtained for IGF-II/M6P and IGF-I receptors in intestinal epithelial membranes and other tissues. Furthermore, the IGF-II/M6P receptor antibody no. 3637 completely inhibited the association of [125I]IGF-II with receptor protein but nonimmune antibody did not, providing additional evidence for the presence of the IGF-II/M6P receptor in the rat gut. Also, analysis of the IGF-II/M6P receptor by immunoblotting using antiserum no. 3637 identified a specific band of mol wt 220.000 throughout the gastrointestinal tract with the highest content of immunoreactivity being present in colon and ileum.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 0013-7227
e-ISSN 1945-7170
Zeitschrift Endocrinology
Quellenangaben Band: 129, Heft: , Seiten: 1769-1778 Artikelnummer: , Supplement: ,
Verlag Endocrine Society
Verlagsort Chevy Chase, Md.
Begutachtungsstatus Peer reviewed