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Wagner, G.* ; Stettmaier, K. ; Bors, W. ; Sies, H.* ; Wagner, E.M.* ; Reuter, A.* ; Weiher, H.*

Enhanced Gamma-Glutamyl Transpeptidase Expression and Superoxide Production in Mpv17-/- Glomerulosclerosis Mice.

Biol. Chem. 382, 1019-1025 (2001)
Open Access Green as soon as Postprint is submitted to ZB.
Recently, gamma-glutamyl transpeptidase, which initiates cleavage of extracellular glutathione, has been shown to promote oxidative damage to cells. Here we examined a murine disease model of glomerulosclerosis, involving loss of the Mpv17 gene coding for a peroxisomal protein. In Mpv17-/- cells, enzyme activity and mRNA expression (examined by quantitative RT-PCR) of membrane-bound gamma-glutamyl transpeptidase were increased, while plasma glutathione peroxidase and superoxide dismutase levels were lowered. Superoxide anion production in these cells was increased as documented by electron spin resonance spectroscopy. In the presence of Mn(III)tetrakis(4-benzoic acid)porphyrin, the activities of gamma-glutamyl transpeptidase and plasma glutathione peroxidase were unchanged, suggesting a relationship between enzyme expression and the amount of reactive oxygen species. Inhibition of gamma-glutamyl transpeptidase by acivicin reverted the lowered plasma glutathione peroxidase and superoxide dismutase activities, indicating reciprocal control of gene expression for these enzymes.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Gene expression Glomerulopathy Glutathione cycle Reactive oxygen Transgenic mice
ISSN (print) / ISBN 1431-6730
e-ISSN 1437-4315
Quellenangaben Volume: 382, Issue: 7, Pages: 1019-1025 Article Number: , Supplement: ,
Publisher de Gruyter
Reviewing status Peer reviewed