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Izzo, A.* ; Regnard, C.* ; Morales, V.* ; Kremmer, E. ; Becker, P.B.*

Structure-function analysis of the RNA helicase maleless.

Nucleic Acids Res. 36, 950-962 (2008)
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Loss of function of the RNA helicase maleless (MLE) in Drosophila melanogaster leads to male-specific lethality due to a failure of X chromosome dosage compensation. MLE is presumably involved in incorporating the non-coding roX RNA into the dosage compensation complex (DCC), which is an essential but poorly understood requirement for faithful targeting of the complex to the X chromosome. Sequence comparison predicts several RNA-binding domains in MLE but their properties have not been experimentally verified. We evaluated the RNA-binding characteristics of these conserved motifs and their contributions to RNA-stimulated ATPase activity, to helicase activity, as well as to the targeting of MLE to the nucleus and to the X chromosome territory. We find that RB2 is the dominant, conditional RNA-binding module, which is indispensable for ATPase and helicase activity whereas the N-terminal RB1 motif does not bind RNA, but is involved in targeting MLE to the X chromosome. The C-terminal domain containing a glycine-rich heptad repeat adds potential dimerization and RNA-binding surfaces which are not required for helicase activity.
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Publication type Article: Journal article
Document type Scientific Article
Keywords DOSAGE COMPENSATION COMPLEX; HEPATITIS-C VIRUS; MALE X-CHROMOSOME; MSL COMPLEX; DROSOPHILA MALELESS; RICH DOMAIN; ROX RNAS; PROTEIN; TRANSLOCATION; LOCALIZATION
ISSN (print) / ISBN 0305-1048
e-ISSN 1362-4962
Quellenangaben Volume: 36, Issue: 3, Pages: 950-962 Article Number: , Supplement: ,
Publisher Oxford University Press
Reviewing status Peer reviewed