as soon as is submitted to ZB.
Gene editing in one-cell embryos by zinc-finger and TAL nucleases.
In: Current Protocols in Mouse Biology. Weinheim: Wiley-VCH, 2013. 347-364 ( ; 2)
Gene targeting by sequence-specific nucleases in one-cell embryos provides an expedited mutagenesis approach in rodents. This technology has been recently established to create knockout and knockin mutants through sequence deletion or sequence insertion. This article provides protocols for the preparation and microinjection of nuclease mRNA and targeting vector DNA into fertilized mouse eggs. Furthermore, we provide guidelines for genotyping the desired mouse mutants.
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Publication type Article: Edited volume or book chapter
Keywords pronucleus injection; gene targeting; mouse mutant; zinc-finger nuclease; TAL nuclease; homologous recombination
Institute(s) Institute of Developmental Genetics (IDG)