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Bauer, M.* ; Meyer, M.* ; Sautter, J.* ; Gasser, T.* ; Ueffing, M. ; Widmer, H.R.*

Liposome-mediated gene transfer to fetal human ventral mesencephalic explant cultures.

Neurosci. Lett. 308, 169-172 (2001)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The feasibility of non-viral gene transfer using liposomes is described for human fetal nigral tissue. Ventral mesencephalic explants from 6 to 12 week old fetuses were grown as free-floating roller tube cultures. For the transfection, a vector coding for beta -galactosidase driven by the Rous Sarcoma Virus promoter was used. The developmental stage of the human tissue, time in vitro and the amount of vector DNA used significantly influenced the transfection efficiency. Optimal transfection results were obtained with tissue from a 10 week old fetus, cultured for 4 days and transfected with mixtures containing 4 mug vector DNA. Histological analysis suggested that a specific population of ventral mesencephalic precursor cells were the target for the gene transfer. This finding might have implications for gene delivery and cell replacement strategies in Parkinson's disease.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter human; development; ventral mesencephalon; tissue culture; gene transfer; liposomes; progenitor cells; dopamine; CENTRAL-NERVOUS-SYSTEM; STEM-CELLS; DOPAMINERGIC-NEURONS; RAT-BRAIN; EXPRESSION; SURVIVAL; MODEL; DNA
ISSN (print) / ISBN 0304-3940
e-ISSN 0304-3940
Zeitschrift Neuroscience Letters
Quellenangaben Band: 308, Heft: 3, Seiten: 169-172 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed