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Liposome-mediated gene transfer to fetal human ventral mesencephalic explant cultures.
Neurosci. Lett. 308, 169-172 (2001)
The feasibility of non-viral gene transfer using liposomes is described for human fetal nigral tissue. Ventral mesencephalic explants from 6 to 12 week old fetuses were grown as free-floating roller tube cultures. For the transfection, a vector coding for beta -galactosidase driven by the Rous Sarcoma Virus promoter was used. The developmental stage of the human tissue, time in vitro and the amount of vector DNA used significantly influenced the transfection efficiency. Optimal transfection results were obtained with tissue from a 10 week old fetus, cultured for 4 days and transfected with mixtures containing 4 mug vector DNA. Histological analysis suggested that a specific population of ventral mesencephalic precursor cells were the target for the gene transfer. This finding might have implications for gene delivery and cell replacement strategies in Parkinson's disease.
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Publication type Article: Journal article
Document type Scientific Article
Keywords human; development; ventral mesencephalon; tissue culture; gene transfer; liposomes; progenitor cells; dopamine; CENTRAL-NERVOUS-SYSTEM; STEM-CELLS; DOPAMINERGIC-NEURONS; RAT-BRAIN; EXPRESSION; SURVIVAL; MODEL; DNA
ISSN (print) / ISBN 0304-3940
Journal Neuroscience Letters
Quellenangaben Volume: 308, Issue: 3, Pages: 169-172
Reviewing status Peer reviewed
Institute(s) Institute of Human Genetics (IHG)