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Möller, W. ; Gibson, N.* ; Geiser, M.* ; Pokrehl, S.* ; Wenk, A. ; Takenaka, S. ; Schmid, O. ; Bulgheroni, A.* ; Simonelli, F.* ; Kozempel, J.* ; Holzwarth, U.* ; Wigge, C.* ; Eigeldinger-Berthou, S.* ; Mädler, L.* ; Kreyling, W.G.

Gold nanoparticle aerosols for rodent inhalation and translocation studies.

J. Nanopart. Res. 15:1574 (2013)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The intensive use of nano-sized particles in many different applications necessitates studies on their risk assessment as there are still open questions on their safe handling and utilization. For reliable risk assessment, the interaction of nanoparticles (NP) with biological systems after various routes of exposure needs to be investigated using well-characterized NP. We report here on the generation of gold-NP (Au-NP) aerosols for inhalation studies with the spark ignition technique, and their characterization in terms of chemical composition, physical structure, morphology, and specific surface area, and on interaction with lung tissues and lung cells after 1 h inhalation by mice. The originally generated agglomerated Au-NP were converted into compact spherical Au-NP by thermal annealing at 600 degrees C, providing particles of similar mass, but different size and specific surface area. Since there are currently no translocation data available on inhaled Au-NP in the 10-50 nm diameter range, the emphasis was to generate NP as small as 20 nm for inhalation in rodents. For anticipated in vivo systemic translocation and dosimetry analyses, radiolabeled Au-NP were created by proton irradiating the gold electrodes of the spark generator, thus forming gamma ray emitting Au-195 with 186 days half-life, allowing long-term biokinetic studies. The dissolution rate of Au-195 from the NP was below detection limits. The highly concentrated, polydisperse Au-NP aerosol (1-2 x 10(7) NP/cm(3)) proved to be constant over several hours in terms of its count median mobility diameter, its geometric standard deviation and number concentration. After collection on filters particles can be re-suspended and used for instillation or ingestion studies.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter gold nanoparticels, spark ignition; chain aggregate/agglomerate; proton irradiation; au-195 radiolabel; x-ray diffraction; Ultrafine Particles ; Oxide Nanoparticle ; Nanotoxicology ; Nanomedicine ; Inflammation ; Deposition ; Lung ; Rats ; Macrophages ; Generation
ISSN (print) / ISBN 1388-0764
e-ISSN 1572-896X
Quellenangaben Band: 15, Heft: 4, Seiten: , Artikelnummer: 1574 Supplement: ,
Verlag Springer
Begutachtungsstatus Peer reviewed