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Pesic, M.* ; Bartholomäus, I.* ; Kyratsous, N.I.* ; Heissmeyer, V. ; Wekerle, H.* ; Kawakami, N.*

2-photon imaging of phagocyte-mediated T cell activation in the CNS.

J. Clin. Invest. 123, 1192-1201 (2013)
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Autoreactive T cells can infiltrate the CNS to cause disorders such as multiple sclerosis. In order to visualize T cell activation in the CNS, we introduced a truncated fluorescent derivative of nuclear factor of activated T cells (NFAT) as a real-time T cell activation indicator. In experimental autoimmune encephalomyelitis, a rat model of multiple sclerosis, we tracked T cells interacting with structures of the vascular blood-brain barrier (BBB). 2-photon imaging documented the cytoplasmic-nuclear translocation of fluorescent NFAT, indicative of calcium-dependent activation of the T cells in the perivascular space, but not within the vascular lumen. The activation was related to contacts with the local antigen-presenting phagocytes and was noted only in T cells with a high pathogenic potential. T cell activation implied the presentation of an autoantigen, as the weakly pathogenic T cells, which remained silent in the untreated hosts, were activated upon instillation of exogenous autoantigen. Activation did not cogently signal long-lasting arrest, as individual T cells were able to sequentially contact fresh APCs. We propose that the presentation of local autoantigen by BBB-associated APCs provides stimuli that guide autoimmune T cells to the CNS destination, enabling them to attack the target tissue.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Experimental Autoimmune Encephalomyelitis ; Transcription Factor Nfat1 ; Immunological Synapses ; Dendritic Cells ; Antigen ; Dephosphorylation ; Accumulation ; Calcineurin ; Determines ; Migration
ISSN (print) / ISBN 0021-9738
e-ISSN 1558-8238
Quellenangaben Band: 123, Heft: 3, Seiten: 1192-1201 Artikelnummer: , Supplement: ,
Verlag American Society of Clinical Investigation
Begutachtungsstatus Peer reviewed