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Sela, G.* ; Razansky, D. ; Shoham, S.*

Multimodal optoacoustic and multiphoton fluorescence microscopy.

In: Progress in biomedical optics and imaging ; 14 (SPIE Photons Plus Ultrasound: Imaging and Sensing 2013, San Francisco USA, 3-5 February 2013). Washington, USA: Society of Photo-Optical Instrumentation Engineers, 2013.:85812H (Proc. SPIE ; 8581)
Multiphoton microscopy is a powerful imaging modality that enables structural and functional imaging with cellular and sub-cellular resolution, deep within biological tissues. Yet, its main contrast mechanism relies on extrinsically administered fluorescent indicators. Here we developed a system for simultaneous multimodal optoacoustic and multiphoton fluorescence 3D imaging, which attains both absorption and fluorescence-based contrast by integrating an ultrasonic transducer into a two-photon laser scanning microscope. The system is readily shown to enable acquisition of multimodal microscopic images of fluorescently labeled targets and cell cultures as well as intrinsic absorption-based images of pigmented biological tissue. During initial experiments, it was further observed that that detected optoacoustically-induced response contains low frequency signal variations, presumably due to cavitation-mediated signal generation by the high repetition rate (80MHz) near IR femtosecond laser. The multimodal system may provide complementary structural and functional information to the fluorescently labeled tissue, by superimposing optoacoustic images of intrinsic tissue chromophores, such as melanin deposits, pigmentation, and hemoglobin or other extrinsic particle or dye-based markers highly absorptive in the NIR spectrum.
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Publication type Article: Conference contribution
Keywords Absorption ; Cavitation ; Femtosecond phenomena ; Laser scanners ; Luminescence ; Microscopes ; Multiphoton fluorescence microscopy ; Near infrared ; Particles ; Photons
ISBN 9780819493507
Conference Title SPIE Photons Plus Ultrasound: Imaging and Sensing 2013, San Francisco USA, 3-5 February 2013
Proceedings Title Progress in biomedical optics and imaging ; 14
Quellenangaben Volume: 8581, Issue: , Pages: , Article Number: 85812H Supplement: ,
Series Proc. SPIE
Publisher Society of Photo-Optical Instrumentation Engineers
Publishing Place Washington, USA