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Padeken, J.* ; Mendiburo, M.J.* ; Chlamydas, S.* ; Schwarz, H.J.* ; Kremmer, E. ; Heun, P.*

The nucleoplasmin homolog NLP mediates centromere clustering and anchoring to the nucleolus.

Mol. Cell 50, 236-249 (2013)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Centromere clustering during interphase is a phenomenon known to occur in many different organisms and cell types, yet neither the factors involved nor their physiological relevance is well understood. Using Drosophila tissue culture cells and flies, we identified a network of proteins, including the nucleoplasmin-like protein (NLP), the insulator protein CTCF, and the nucleolus protein Modulo, to be essential for the positioning of centromeres. Artificial targeting further demonstrated that NLP and CTCF are sufficient for clustering, while Modulo serves as the anchor to the nucleolus. Centromere clustering was found to depend on centric chromatin rather than specific DNA sequences. Moreover, unclustering of centromeres results in the spatial destabilization of pericentric heterochromatin organization, leading to partial defects in the silencing of repetitive elements, defects during chromosome segregation, and genome instability.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Position-effect Variegation ; Drosophila-melanogaster ; Nuclear Periphery ; Chromatin ; Ctcf ; Protein ; Heterochromatin ; Nucleophosmin ; Localization ; Organization
ISSN (print) / ISBN 1097-2765
e-ISSN 1097-4164
Journal Molecular Cell
Quellenangaben Volume: 50, Issue: 2, Pages: 236-249 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed