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Schneider, K.* ; Fuchs, C. ; Dobay, A.* ; Rottach, A.* ; Qin, W.H.* ; Wolf, P.* ; Alvarez-Castro, J.M.* ; Nalaskowski, M.M.* ; Kremmer, E. ; Schmid, V.* ; Leonhardt, H.* ; Schermelleh, L.*

Dissection of cell cycle-dependent dynamics of Dnmt1 by FRAP and diffusion-coupled modeling.

Nucleic Acids Res. 41, 4860-4876 (2013)
Verlagsversion Volltext DOI
Open Access Gold
Creative Commons Lizenzvertrag
DNA methyltransferase 1 (Dnmt1) reestablishes methylation of hemimethylated CpG sites generated during DNA replication in mammalian cells. Two subdomains, the proliferating cell nuclear antigen (PCNA)-binding domain (PBD) and the targeting sequence (TS) domain, target Dnmt1 to the replication sites in S phase. We aimed to dissect the details of the cell cycle-dependent coordinated activity of both domains. To that end, we combined super-resolution 3D-structured illumination microscopy and fluorescence recovery after photobleaching (FRAP) experiments of GFP-Dnmt1 wild type and mutant constructs in somatic mouse cells. To interpret the differences in FRAP kinetics, we refined existing data analysis and modeling approaches to (i) account for the heterogeneous and variable distribution of Dnmt1-binding sites in different cell cycle stages; (ii) allow diffusion-coupled dynamics; (iii) accommodate multiple binding classes. We find that transient PBD-dependent interaction directly at replication sites is the predominant specific interaction in early S phase (residence time T-res 10 s). In late S phase, this binding class is taken over by a substantially stronger (T-res similar to 22 s) TS domain-dependent interaction at PCNA-enriched replication sites and at nearby pericentromeric heterochromatin subregions. We propose a two-loading-platform-model of additional PCNA-independent loading at postreplicative, heterochromatic Dnmt1 target sites to ensure faithful maintenance of densely methylated genomic regions.
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Icb_biostatistics Icb_Latent Causes
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Embryonic Stem-cells ; Dna Methyltransferase 1 ; Recombinant Human Dna ; Hemi-methylated Dna ; Living Cells ; Fluorescence Recovery ; In-vivo ; De-novo ; Structured Illumination ; Anomalous Diffusion
ISSN (print) / ISBN 0305-1048
e-ISSN 1362-4962
Quellenangaben Band: 41, Heft: 9, Seiten: 4860-4876 Artikelnummer: , Supplement: ,
Verlag Oxford University Press
Begutachtungsstatus Peer reviewed