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Molecular cartography in acute Chlamydia pneumoniae infections - a non-targeted metabolomics approach.
Anal. Bioanal. Chem. 405, 5119-5131 (2013)
Infections with Chlamydia pneumoniae cause several respiratory diseases, such as community-acquired pneumonia, bronchitis or sinusitis. Here, we present an integrated non-targeted metabolomics analysis applying ultra-high-resolution mass spectrometry and ultra-performance liquid chromatography mass spectrometry to determine metabolite alterations in C. pneumoniae-infected HEp-2 cells. Most important permutations are elaborated using uni- and multivariate statistical analysis, logD retention time regression and mass defect-based network analysis. Classes of metabolites showing high variations upon infection are lipids, carbohydrates and amino acids. Moreover, we observed several non-annotated compounds as predominantly abundant after infection, which are promising biomarker candidates for drug-target and diagnostic research.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Mass spectronomy; Host-pathogen interactions; Liquid chromatography; HOST-CELL; MASS-SPECTROMETRY; SYSTEMS BIOLOGY; INTRACELLULAR SURVIVAL; GLUCOSE-METABOLISM; PLASMA-MEMBRANE; ORGANIC-MATTER; TRACHOMATIS; INCLUSION; PATHOGEN
ISSN (print) / ISBN 1618-2642
Quellenangaben Volume: 405, Issue: 15, Pages: 5119-5131
Publishing Place Heidelberg
Reviewing status Peer reviewed
Institute(s) Research Unit Analytical BioGeoChemistry (BGC)