Clinical chemistry and other laboratory tests on mouse plasma and serum.
Curr. Protoc. Mouse Biol. 3, 69-100 (2013)
Besides hematological analyses, many other parameters, including clinical chemistry and endocrinological values, can be determined from mouse blood samples. For most of these tests, plasma or serum samples are used. Data obtained by these investigations provide indications of genotype effects on metabolism and organ functions. Here we describe in detail the considerations that have to be taken into account to get adequate samples for plasma or serum analyses and the recommended sample processing for different investigations. Furthermore, we describe established methods used in the German Mouse Clinic (GMC) to determine clinical chemical parameters; for more in-depth analysis of specific classes of biomarkers, we provide instructions for ELISAs (sandwich and competitive) as well as LC-MS/MS, focusing on markers associated with bone or steroid metabolism in the mouse as working examples.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter mouse; clinical chemistry; steroids; osteocalcin; PINP; ELISA; mass spectrometry
ISSN (print) / ISBN 2161-2617
Zeitschrift Current Protocols of Mouse Biology
Quellenangaben Band: 3, Seiten: 69-100
Institut(e) Institute of Experimental Genetics (IEG)