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Drosophila miR-277 controls branched-chain amino acid catabolism and affects lifespan.
RNA Biol. 10, 1042-1056 (2013)
Verlagsversion Volltext DOI
Development, growth and adult survival are coordinated with available metabolic resources, ascertaining that the organism responds appropriately to environmental conditions. MicroRNAs are short (21-23 nt) regulatory RNAs that confer specificity on the RNA-induced silencing complex (RISC) to inhibit a given set of mRNA targets. We profiled changes in miRNA expression during adult life in Drosophila melanogaster and determined that miR-277 is downregulated during adult life. Molecular analysis revealed that this miRNA controls branched-chain amino acid (BCAA) catabolism and as a result it can modulate the activity of the TOR kinase, a central growth regulator, in cultured cells. Metabolite analysis in cultured cells as well as flies suggests that the mechanistic basis may be an accumulation of branched-chain α-keto-acids (BCKA), rather than BCAAs, thus avoiding potentially detrimental consequences of increased branched chain amino acid levels on e.g., translational fidelity. Constitutive miR-277 expression shortens lifespan and is synthetically lethal with reduced insulin signaling, indicating that metabolic control underlies this phenotype. Transgenic inhibition with a miRNA sponge construct also shortens lifespan, in particular on protein-rich food. Thus, optimal metabolic adaptation appears to require tuning of cellular BCAA catabolism by miR-277.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter ageing; diabetes; longevity; maple syrup urine disease (MSUD); metabolic syndrome; miRNA; miRNA target validation; Microrna Target Predictions ; Skeletal-muscle ; Mitochondrial Biogenesis ; Dehydrogenase Complex ; Insulin-resistance ; Signaling Pathway ; Protein-synthesis ; Mammalian-cells ; Rna-synthesis ; Melanogaster
ISSN (print) / ISBN 1547-6286
Zeitschrift RNA Biology
Quellenangaben Band: 10, Heft: 6, Seiten: 1042-1056
Verlag Landes Bioscience
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Analytical BioGeoChemistry (BGC)