PuSH - Publication Server of Helmholtz Zentrum München

Niehaus, E.-M.* ; Kleigrewe, K.* ; Wiemann, P.* ; Studt, L.* ; Sieber, C.M.K. ; Connolly, L.R.* ; Freitag, M.* ; Güldener, U. ; Tudzynski, B.* ; Humpf, H.-U.*

Genetic manipulation of the Fusarium fujikuroi fusarin gene cluster yields insight into the complex regulation and fusarin biosynthetic pathway.

Chem. Biol. 20, 1055-1066 (2013)
Open Access Green as soon as Postprint is submitted to ZB.
In this work, the biosynthesis and regulation of the polyketide synthase/nonribosomal peptide synthetase (PKS/NRPS)-derived mutagenic mycotoxin fusarin C was studied in the fungus Fusarium fujikuroi. The fusarin gene cluster consists of nine genes (fus1-fus9) that are coexpressed under high-nitrogen and acidic pH conditions. Chromatin immunoprecipitation revealed a correlation between high expression and enrichment of activating H3K9-acetylation marks under inducing conditions. We provide evidence that only four genes are sufficient for the biosynthesis. The combination of genetic engineering with nuclear magnetic resonance and mass-spectrometry-based structure elucidation allowed the discovery of the putative fusarin biosynthetic pathway. Surprisingly, we indicate that PKS/NRPS releases its product with an open ring structure, probably as an alcohol. Our data indicate that 2-pyrrolidone ring closure, oxidation at C-20, and, finally, methylation at C-20 are catalyzed by Fus2, Fus8, and Fus9, respectively.
Additional Metrics?
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Keywords Structure Elucidation; Secondary Metabolism; Moniliforme; Identification; Fungi; Verticillioides; Mycotoxin; Mutagen; Acid; Polyketides
ISSN (print) / ISBN 1074-5521
e-ISSN 1879-1301
Quellenangaben Volume: 20, Issue: 8, Pages: 1055-1066 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed