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Alqueres, S. ; Meneses, C.* ; Rouws, L.* ; Rothballer, M. ; Baldani, I.* ; Schmid, M. ; Hartmann, A.

The bacterial superoxide dismutase and glutathione reductase are crucial for endophytic colonization of rice roots by Gluconacetobacter diazotrophicus PAL5.

Mol. Plant Microbe Interact. 26, 937-945 (2013)
DOI
Gluconacetobacter diazotrophicus is an aerobic diazotrophic plant-growth-promoting bacterium isolated from different gramineous plants. We showed that reactive oxygen species (ROS) were produced at early stages of rice root colonization, a typical plant defense response against pathogens. The transcription of the pathogen-related-10 gene of the jasmonic acid (JA) pathway but not of the PR-1 gene of the salicylic acid pathway was activated by the endophytic colonization of rice roots by G. diazotrophicus strain PALS. Quantitative polymerase chain reaction analyses showed that, at early stages of colonization, the bacteria upregulated the transcript levels of ROS-detoxifying genes such as superoxide dismutase (SOD) and glutathione reductase (GR). To proof the role of ROS-scavenging enzymes in the colonization and interaction process, transposon insertion mutants of the SOD and GR genes of strain PALS were constructed. The SOD and GR mutants were unable to efficiently colonize the roots, indicated by the decrease of tightly root-associated bacterial cell counts and endophytic colonization and by fluorescence in situ hybridization analysis. Interestingly, the mutants did not induce the PR-10 of the JA-pathway, probably due to the inability of endophytic colonization. Thus, ROS-scavenging enzymes of G. diazotrophicus strain PALS play an important role in the endophytic colonization of rice plants.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Biological Nitrogen-fixation ; Induced Systemic Resistance ; Oxidative Burst ; Plant-growth ; Acetobacter-diazotrophicus ; Medicago-truncatula ; Disease Resistance ; Sinorhizobium-meliloti ; Greenhouse Conditions ; Beneficial Microbes
ISSN (print) / ISBN 0894-0282
e-ISSN 1943-7706
Quellenangaben Band: 26, Heft: 8, Seiten: 937-945 Artikelnummer: , Supplement: ,
Verlag American Phytopathological Society
Begutachtungsstatus Peer reviewed