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Frommberger, M. ; Zürbig , P.* ; Jantos, J.* ; Krahn, T.* ; Mischak, H.* ; Pich, A.* ; Just, I.* ; Schmitt-Kopplin, P. ; Schiffer, E.*

Peptidomic analysis of rat urine using capillary electrophoresis coupled to mass spectrometry.

Proteomics Clin. Appl. 1, 650-660 (2007)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
We have established and validated a protocol for the peptidomic analysis of rat urine using CE coupled to MS (CE-MS). In the first experiments, the reproducibility of the CE-MS set-up and of the established preparation procedure were assessed. To establish a first rat urinary peptidome map, samples were also analyzed using CE-FT-ICR. The subsequent analysis of independent samples from two different strains (WISTAR and CD) indicated strain-specific differences, which were validated in a blinded assessment. MS/MS revealed the presence of specific fragments from well-known urinary rat peptides, such as collagens, alpha-1-antitrypsin, and serum albumin. The CE-MS-based peptidomics platform may provide novel insights into body fluids of animal models, such as rat or mice. Together with peptide identification, the technology appears to be an excellent, complimentary, and non-invasive tool to analyze toxicological or other (patho)physiological effects of pharmaceutical compounds in animal models.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Biomarker; CE-MS; Rat; Urine
ISSN (print) / ISBN 1862-8346
e-ISSN 1862-8354
Quellenangaben Band: 1, Heft: 7, Seiten: 650-660 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Weinheim
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Ecological Chemistry (IOEC)