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The impact of blood on liver metabolite profiling - a combined metabolomic and proteomic approach.

Biomed. Chromatogr. 28, 231-240 (2014)
Verlagsversion Volltext DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Metabolomics has entered the well-established omic sciences as it is an indispensable information resource to achieve a global picture of biological systems. The aim of the present study was to estimate the influence of blood removal from mice liver as part of sample preparation for metabolomic and proteomic studies. For this purpose, perfused mice liver tissue (i.e. with blood removed) and unperfused mice liver tissue (i.e. containing blood) were compared by two-dimensional gas chromatography time of flight mass spectrometry (GC × GC-TOFMS) for the metabolomic part, and by liquid chromatography tandem mass spectrometry (LC-MS/MS) for the proteomic part. Our data showed significant differences between the unperfused and perfused liver tissue samples. Furthermore, we also observed an overlap of blood and tissue metabolite profiles in our data, suggesting that the perfusion of liver tissue prior to analysis is beneficial for an accurate metabolic profile of this organ
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter blood; gas chromatography mass spectrometry; liquid chromatography tandem mass spectrometry; metabolomics; mice liver perfusion; proteomics; 2-dimensional Gas-chromatography; Flight Mass-spectrometry; Fatty Liver; Tissue-extracts; Mice; Plasma; Time; Metabonomics; Steatosis; Disease
ISSN (print) / ISBN 0269-3879
e-ISSN 1099-0801
Quellenangaben Band: 28, Heft: 2, Seiten: 231-240 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Hoboken
Begutachtungsstatus Peer reviewed