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Engert, S. ; Burtscher, I. ; Kalali, B.* ; Gerhard, M.* ; Lickert, H.

The Sox17CreERT2 knock-in mouse line displays spatiotemporal activation of Cre recombinase in distinct Sox17 lineage progenitors.

Genesis 51, 793-802 (2013)
Open Access Green as soon as Postprint is submitted to ZB.
The HMG-box transcription factor Sox17 is essential for endoderm formation, vascular development, and definitive hematopoiesis. To investigate the fate of distinct Sox17-expressing progenitor cells in a spatiotemporal manner, we generated a hormone-inducible CreERT2 knock-in mouse line. By homologous recombination we fused a codon improved, ligand-dependent estrogen receptor Cre recombinase by an intervening viral T2A sequence for co-translational cleavage to the 3' coding region of Sox17. Induction of Cre activity by administration of tamoxifen at defined time points of early mouse development and subsequent genetic lineage tracing confirmed the inducibility and tissue specificity of Cre recombination. Furthermore, Cre activity could be selectively induced in extra-embryonic and embryonic endoderm lineages, the primitive gut tube, and in endothelial cells of the vascular system as well as in the hemogenic endothelium of the dorsal aorta. The Sox17CreERT2 mouse line therefore represents a new tool for genetic lineage tracing in a tissue-specific manner and in addition enables lineage-restricted functional analysis.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Sox17; CreERT2; Endoderm; Vasculate; Hematopoietic stem cells; Endoderm Formation ; Redundant Roles ; Stem-cells ; Mice ; Expression ; Genes
ISSN (print) / ISBN 1526-954X
e-ISSN 1526-968X
Quellenangaben Volume: 51, Issue: 11, Pages: 793-802 Article Number: , Supplement: ,
Publisher Wiley
Reviewing status Peer reviewed