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High-resolution optoacoustic mesoscopy with a 24 MHz multidetector translate-rotate scanner.

J. Biomed. Opt. 18:106005 (2013)
Open Access Green as soon as Postprint is submitted to ZB.
Optoacoustic (photoacoustic) mesoscopy aims at high-resolution optical imaging of anatomical, functional, and cellular parameters at depths that go well beyond those of optical-resolution optical or optoacoustic microscopy i.e., reaching several millimeters in depth. The approach utilizes tomography to achieve ultrasonic-diffraction resolution and operates at high-ultrasound frequencies (20 to 200 MHz) induced by few-nanosecond laser pulse excitation of tissues. We investigated here the performance of optoacoustic mesoscopy implemented at 24 MHz center frequency and its ability to resolve optical absorption contrast in the mouse kidney ex vivo. The developed system achieved better than 30 μm in-plane resolution and 110 μm elevation resolution over a cylindrical volume of 9-mm diameter and 9-mm height. This unprecedented combination of resolution and depth was achieved by implementing a translate-rotate detection geometry and by tomographic reconstruction. The approach yielded images of optically absorbing structures with a level of detail never-before visualized in an intact mouse kidney and allows insights into their unperturbed architecture. We discuss the ability to offer multispectral acquisitions and enable in vivo imaging.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Optoacoustic ; Mesoscopic Scale ; Linear Array ; Computed Tomography ; High-frequency Ultrasound; Ultrasound Array ; 1-3 Composite ; Linear-array ; In-vivo ; Tomography ; Reconstruction ; Performance ; Microscopy ; Inversion
ISSN (print) / ISBN 1083-3668
e-ISSN 1560-2281
Quellenangaben Volume: 18, Issue: 10, Pages: , Article Number: 106005 Supplement: ,
Publisher SPIE
Reviewing status Peer reviewed