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Anchoring and ordering NGS contig assemblies by population sequencing (POPSEQ).
Plant J. 76, 718-727 (2013)
Next-generation whole-genome shotgun assemblies of complex genomes are highly useful, but fail to link nearby sequence contigs with each other or provide a linear order of contigs along individual chromosomes. Here, we introduce a strategy based on sequencing progeny of a segregating population that allows de novo production of a genetically anchored linear assembly of the gene space of an organism. We demonstrate the power of the approach by reconstructing the chromosomal organization of the gene space of barley, a large, complex and highly repetitive 5.1 Gb genome. We evaluate the robustness of the new assembly by comparison to a recently released physical and genetic framework of the barley genome, and to various genetically ordered sequence-based genotypic datasets. The method is independent of the need for any prior sequence resources, and will enable rapid and cost-efficient establishment of powerful genomic information for many species.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Next-generation Sequencing ; Genome Assembly ; Genetic Mapping ; Barley ; Hordeum Vulgare ; Population Sequencing ; Technical Advance; Maize Genome ; Draft Genome ; Wheat ; Map ; Association