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Fellner, L.* ; Bechtel, N.* ; Witting, M.A. ; Simon, S.* ; Schmitt-Kopplin, P. ; Keim, D.* ; Scherer, S.* ; Neuhaus, K.*

Phenotype of htgA (mbiA), a recently evolved orphan gene of Escherichia coli and Shigella, completely overlapping in antisense to yaaW.

FEMS Microbiol. Lett. 350, 57-64 (2014)
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Overlapping embedded genes, such as htgA/yaaW, are assumed to be rare in prokaryotes. In Escherichia coli O157:H7, gfp fusions of both promoter regions revealed activity and transcription start sites could be determined for both genes. Both htgA and yaaW were inactivated strand specifically by introducing a stop codon. Both mutants exhibited differential phenotypes in biofilm formation and metabolite levels in a nontargeted analysis, suggesting that both are functional despite YaaW but not HtgA could be expressed. While yaaW is distributed all over the Gammaproteobacteria, an overlapping htgA-like sequence is restricted to the Escherichia-Klebsiella clade. Full-length htgA is only present in Escherichia and Shigella, and htgA showed evidence for purifying selection. Thus, htgA is an interesting case of a lineage-specific, nonessential and young orphan gene.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter htgA-yaaW; functional characterization; overlapping genes; Suicide Vector; Proteins; Mutations; Selection; Origin; Probe
ISSN (print) / ISBN 0378-1097
e-ISSN 1574-6968
Quellenangaben Band: 350, Heft: 1, Seiten: 57-64 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed