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Besong Agbo, D.* ; Klafki, H.* ; Poschmann, G.* ; Genius, J.* ; Janßen, C.* ; Stühler, K.* ; Wurst, W. ; Meyer, H.E.* ; Klingenspor, M.* ; Wiltfang, J.*

Development of a capillary isoelectric focusing immunoassay to measure DJ-1 isoforms in biological samples.

Anal. Biochem. 443, 197-204 (2013)
DOI Verlagsversion bestellen
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
We report on the development of a novel assay protocol for the separation and detection of charge isoforms of DJ-1 in biological samples by automated capillary isoelectric focusing followed by immunological detection. DJ-1 (PARK7) is considered as a biomarker candidate for Parkinson's disease and may potentially support the differentiation of clinical subtypes of the disease. The new method allows for separation and subsequent relative quantitative comparison of different isoforms of DJ-1 in biological samples. The assay was successfully applied to the analysis of DJ-1 isoform patterns in brains from mice subjected to normal or high-fat diet and revealed statistically significant group differences. Furthermore, in a pooled and concentrated sample of human cerebrospinal fluid that was depleted of albumin and immunoglobulin G, four different charge variants of DJ-1 could be detected. Taken together, the capillary isoelectric focusing immunoassay for DJ-1 represents a promising tool that may ultimately serve in clinical biomarker studies.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Biomarker; CSF; DJ-1; Immunoassay; Isoelectric focusing; PARK7; Parkinsons-disease ; 2-dimensional Electrophoresis ; Cerebrospinal-fluid ; Biomarkers ; Cells
ISSN (print) / ISBN 0003-2697
e-ISSN 1096-0309
Quellenangaben Band: 443, Heft: 2, Seiten: 197-204 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed