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Heinrich, S.* ; Geissen, E.M.* ; Kamenz, J.* ; Trautmann, S.* ; Widmer, C.* ; Drewe, P.* ; Knop, M.* ; Radde, N.* ; Hasenauer, J. ; Hauf, S.*

Determinants of robustness in spindle assembly checkpoint signalling.

Nat. Cell Biol. 15, 1328-1239 (2013)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The spindle assembly checkpoint is a conserved signalling pathway that protects genome integrity. Given its central importance, this checkpoint should withstand stochastic fluctuations and environmental perturbations, but the extent of and mechanisms underlying its robustness remain unknown. We probed spindle assembly checkpoint signalling by modulating checkpoint protein abundance and nutrient conditions in fission yeast. For core checkpoint proteins, a mere 20% reduction can suffice to impair signalling, revealing a surprising fragility. Quantification of protein abundance in single cells showed little variability (noise) of critical proteins, explaining why the checkpoint normally functions reliably. Checkpoint-mediated stoichiometric inhibition of the anaphase activator Cdc20 (Slp1 in Schizosaccharomyces pombe) can account for the tolerance towards small fluctuations in protein abundance and explains our observation that some perturbations lead to non-genetic variation in the checkpoint response. Our work highlights low gene expression noise as an important determinant of reliable checkpoint signalling.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Anaphase-promoting Complex ; Cycle-regulated Genes ; Fission Yeast ; Schizosaccharomyces-pombe ; Mitotic Checkpoint ; Cell-cycle ; Saccharomyces-cerevisiae ; Crystal-structure ; Chromosome Instability ; Biological Noise
ISSN (print) / ISBN 1465-7392
e-ISSN 1476-4679
Zeitschrift Nature Cell Biology
Quellenangaben Band: 15, Heft: 11, Seiten: 1328-1239 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Begutachtungsstatus Peer reviewed