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Enantioseparation and selective detection of D-amino acids by ultra-high-performance liquid chromatography/mass spectrometry in analysis of complex biological samples.

J. Chromatogr. A 1324, 109-114 (2014)
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Open Access Green
The growing scientific attention in the biological function of D-amino acids leads to an increasing analytical interest for enantiomeric amino acid separation, which is still very challenging due to the lack of sufficiently sensitive, high-throughput analytical methods that can cope with often occurring matrix interferences and very low D-amino acid concentrations. Here, enantioseparation can benefit from improved resolution and chromatographic speed offered by modern UHPLC techniques and the precision of MS detection. We developed a RP-UHPLC-QqToF-MS method using pre-column OPA/IBLC derivatization for very precise discrimination of amino acids enantiomers. The method shows a superb sensitivity with limits of detection in the range of several pmol/l. It has neither shown matrix inferences in the tested very complex biological matrices (serum, plasma, urine and gut) nor stability or racemisation problems.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter chiral derivatization; chiral separation; liquid chromatography; mass spectrometry; D-amino acids; complex matrices; OPA-IBLC; L-cysteine Reagents; Mass-spectrometry; O-phthaldialdehyde/3-mercaptopropionic Acid; O-phthaldialdehyde; Derivatives; Derivatization; Behavior; Mammals; Fluids
ISSN (print) / ISBN 0021-9673
e-ISSN 1873-3778
Zeitschrift Journal of Chromatography A
Quellenangaben Band: 1324, Heft: , Seiten: 109-114 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus