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High-resolution metabolite imaging of light and dark treated retina using MALDI-FTICR mass spectrometry.

Proteomics 14, 913-923 (2014)
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Open Access Green
Mass spectrometry imaging (MSI) is a valuable tool for diagnostics and systems biology studies, being a highly sensitive, label-free technique capable of providing comprehensive spatial distribution of different classes of biomolecules. The application of MSI to the study of endogenous compounds has received considerable attention because metabolites are the result of the interactions of a biosystem with its environment. MSI can therefore enhance understanding of disease mechanisms and elucidate mechanisms for biological variation. Here we present the in situ comparative metabolomics imaging data for analyses of light- and dark-treated retina. A wide variety of tissue metabolites were imaged at a high spatial resolution. These include nucleotides, central carbon metabolism pathway intermediates, 2-oxocarboxylic acid metabolism, oxidative phosphorylation, glycerophospholipid metabolism, and cysteine and methionine metabolites. The high lateral resolution enabled the differentiation of retinal layers, allowing determination of the spatial distributions of different endogenous compounds. A number of metabolites demonstrated differences between light and dark conditions. These findings add to the understanding of metabolic activity in the retina. This article is protected by copyright. All rights reserved.
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Publication type Article: Journal article
Document type Scientific Article
Keywords FTICR; MALDI; Mass spectrometry imaging; Metabolomics; Retina; Lens Alpha-crystallin; Outer Retina; Diabetic-retinopathy; Oxygen-consumption; Glucose-metabolism; Macular Degeneration; Spatial-distribution; Mammalian Retina; Inner Retina; Rat Retina
ISSN (print) / ISBN 1615-9853
e-ISSN 1615-9861
Journal Proteomics
Quellenangaben Volume: 14, Issue: 7-8, Pages: 913-923 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Hoboken
Reviewing status Peer reviewed