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Shen, H.* ; Xu, W.* ; Peng, S.* ; Scherb, H. ; She, J.* ; Voigt, K. ; Alamdar, A.* ; Schramm, K.-W.

Pooling samples for "top-down" molecular exposomics research: The methodology.

Environ. Health 13:8 (2014)
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Open Access Gold
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BACKGROUND: Exposomics is the cutting-edge concept of screening the environmental risk factors for disease. In the novel "top-down" approach, we estimate the molecular exposome by measuring all body fluid analytes in a case-controlled study. However, to detect diverse pollutants, a sufficient sample size and multiple analytical methods are required. This may lead to dramatically increased costs and research workload. METHODS: To help reduce complexity, we suggest a sample pooling strategy along with a scheme for combining both general unknown or multi-targeted screening with targeted analysis. The sample pooling method was tested using computer simulations. RESULTS: By comprehensively analysis of pooled samples, it is possible to identify environmental risk factors. Factors are initially screened in the pooled case and control population samples, then in the randomized grouped and pooled case and control subpopulation samples. In the sample grouping, five or more pools were suggested for groups having 30 individuals per pool. CONCLUSIONS: This study suggests that sample pooling is a useful strategy for exposomics research, which provides a hypothesis-free method for pollutant risk screening.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Exposome ; Exposure Biomarker ; Metabolome ; Effect Biomarker ; Case-control Study ; Environmental Pollutants ; Pooled Sample; Pooled Blood-samples; Breast-milk; Exposure; Metabonomics; Association; Chemicals; Genome; Issues
e-ISSN 1476-069x
Quellenangaben Volume: 13, Issue: 1, Pages: , Article Number: 8 Supplement: ,
Publisher BioMed Central
Publishing Place London
Reviewing status