PuSH - Publication Server of Helmholtz Zentrum München

Böhm, A. ; Halama, A. ; Meile, T.* ; Zdichavsky, M.* ; Lehmann, R. ; Weigert, C. ; Fritsche, A. ; Stefan, N. ; Königsrainer, A.* ; Häring, H.-U. ; Hrabě de Angelis, M. ; Adamski, J. ; Staiger, H.

Metabolic signatures of cultured human adipocytes from metabolically healthy versus unhealthy obese individuals.

PLoS ONE 9:e93148 (2014)
Publ. Version/Full Text Volltext DOI
Open Access Gold
Creative Commons Lizenzvertrag
BACKGROUND AND AIMS: Among obese subjects, metabolically healthy and unhealthy obesity (MHO/MUHO) can be differentiated: the latter is characterized by whole-body insulin resistance, hepatic steatosis, and subclinical inflammation. Aim of this study was, to identify adipocyte-specific metabolic signatures and functional biomarkers for MHO versus MUHO. METHODS: 10 insulin-resistant (IR) vs. 10 insulin-sensitive (IS) non-diabetic morbidly obese (BMI >40 kg/m2) Caucasians were matched for gender, age, BMI, and percentage of body fat. From subcutaneous fat biopsies, primary preadipocytes were isolated and differentiated to adipocytes in vitro. About 280 metabolites were investigated by a targeted metabolomic approach intracellularly, extracellularly, and in plasma. RESULTS/INTERPRETATION: Among others, aspartate was reduced intracellularly to one third (p = 0.0039) in IR adipocytes, pointing to a relative depletion of citric acid cycle metabolites or reduced aspartate uptake in MUHO. Other amino acids, already known to correlate with diabetes and/or obesity, were identified to differ between MUHO's and MHO's adipocytes, namely glutamine, histidine, and spermidine. Most species of phosphatidylcholines (PCs) were lower in MUHO's extracellular milieu, though simultaneously elevated intracellularly, e.g., PC aa C32∶3, pointing to increased PC synthesis and/or reduced PC release. Furthermore, altered arachidonic acid (AA) metabolism was found: 15(S)-HETE (15-hydroxy-eicosatetraenoic acid; 0 vs. 120pM; p = 0.0014), AA (1.5-fold; p = 0.0055) and docosahexaenoic acid (DHA, C22∶6; 2-fold; p = 0.0033) were higher in MUHO. This emphasizes a direct contribution of adipocytes to local adipose tissue inflammation. Elevated DHA, as an inhibitor of prostaglandin synthesis, might be a hint for counter-regulatory mechanisms in MUHO. CONCLUSION/INTERPRETATION: We identified adipocyte-inherent metabolic alterations discriminating between MHO and MUHO.
Altmetric
Additional Metrics?
Tags
GAC
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Keywords Type-2 Diabetes-mellitus; Activated Receptor-gamma; Arachidonic-acid; Insulin Sensitivity; 3t3-l1 Adipocytes; Adipose-tissue; Glucose-uptake; Fatty Liver; Cycle Flux; Risk
ISSN (print) / ISBN 1932-6203
Journal PLoS ONE
Quellenangaben Volume: 9, Issue: 4, Pages: , Article Number: e93148 Supplement: ,
Publisher Public Library of Science (PLoS)
Publishing Place Lawrence, Kan.
Reviewing status Peer reviewed