High-throughput phenotypic assessment of cardiac physiology in four commonly used inbred mouse strains.
J. Comp. Physiol. B 184, 763-775 (2014)
Mice with genetic alterations are used in heart research as model systems of human diseases. In the last decade there was a marked increase in the recognition of genetic diversity within inbred mouse strains. Increasing numbers of inbred mouse strains and substrains and analytical variation of cardiac phenotyping methods require reproducible, high-throughput methods to standardize murine cardiovascular physiology. We describe methods for non-invasive, reliable, easy and fast to perform echocardiography and electrocardiography on awake mice. This method can be used for primary screening of the murine cardiovascular system in large-scale analysis. We provide insights into the physiological divergence of C57BL/6N, C57BL/6J, C3HeB/FeJ and 129P2/OlaHsd mouse hearts and define the expected normal values. Our report highlights that compared to the other three strains tested C57BL/6N hearts reveal features of heart failure such as hypertrophy and reduced contractile function. We found several features of the mouse ECG to be under genetic control and obtained several strain-specific differences in cardiac structure and function.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter C57bl/6 ; Echocardiography ; Electrocardiography ; Heart ; Screening; Left-ventricular Function; Integrin-linked Kinase; Heart-rate; Echocardiographic-assessment; Dilated Cardiomyopathy; Conduction System; Gene-expression; Deficient Mice; Interval; Electrocardiogram
ISSN (print) / ISBN 0174-1578
Zeitschrift Journal of Comparative Physiology B
Quellenangaben Band: 184, Heft: 6, Seiten: 763-775