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Quantitative analysis of axonal outgrowth in mice.
Neuromethods 87, 145-162 (2014)
The ability to exert complex locomotor behaviors requires precise guidance of sensory and motor fibers to the extremities, resulting in the formation of precise peripheral networks. Interactions of growing axons with their environment and co-extending nerve fibers have been shown to critically contribute to the establishment of neuronal projections to the limb, governing accurate fasciculation of heterotypic fiber systems and mediating the stereotypic dorsal-ventral guidance decisions of growing motor axons at the base of the limb. Here we provide a detailed methodology to quantitate selective fasciculation of axon tracts at specific choice points as well as guidance fidelity of motor neurons projecting to the dorsal or ventral limb during embryonal development. Immunohistochemical staining of whole-mount embryo preparations was employed to analyze patterned growth of axons towards the plexus region, and selective branching beyond this choice point. Retrograde tracing of motor neurons from the dorsal or ventral limb mesenchyme served to analyze stereotypical dorsal-ventral guidance decisions of motor neurons localized in the medial and lateral aspects of the lateral motor column (LMC). These methods therefore provide a valuable tool to reliably quantify sensory-motor fasciculation and stereotypic guidance events during early embryonal development.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Axon Guidance ; Fasciculation ; Guidance Fidelity ; Retrograde Tracing ; Sensory-motor Circuitry
ISSN (print) / ISBN 1940-6045
Quellenangaben Band: 87, Seiten: 145-162
Institut(e) Institute of Developmental Genetics (IDG)