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van de Weijer, M.L.* ; Bassik, M.C.* ; Luteijn, R.D.* ; Voorburg, C.M.* ; Lohuis, M.A.* ; Kremmer, E. ; Hoeben, R.C.* ; Leproust, E.M.* ; Chen, S.* ; Hoelen, H.* ; Ressing, M.E.* ; Patena, W.* ; Weissman, J.S.* ; McManus, M.T.* ; Wiertz, E.J.* ; Lebbink, R.J.*

A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation.

Nat. Commun. 5:3832 (2014)
Verlagsversion Volltext DOI
Open Access Gold
Misfolded ER proteins are retrotranslocated into the cytosol for degradation via the ubiquitin-proteasome system. The human cytomegalovirus protein US11 exploits this ER-associated protein degradation (ERAD) pathway to downregulate HLA class I molecules in virus-infected cells, thereby evading elimination by cytotoxic T-lymphocytes. US11-mediated degradation of HLA class I has been instrumental in the identification of key components of mammalian ERAD, including Derlin-1, p97, VIMP and SEL1L. Despite this, the process governing retrotranslocation of the substrate is still poorly understood. Here using a high-coverage genome-wide shRNA library, we identify the uncharacterized protein TMEM129 and the ubiquitin-conjugating E2 enzyme UBE2J2 to be essential for US11-mediated HLA class I downregulation. TMEM129 is an unconventional C4C4-type RING finger E3 ubiquitin ligase that resides within a complex containing various other ERAD components, including Derlin-1, Derlin-2, VIMP and p97, indicating that TMEM129 is an integral part of the ER-resident dislocation complex mediating US11-induced HLA class I degradation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Class-i Molecules; Reticulum-associated Degradation; Large Gene Lists; Endoplasmic-reticulum; Membrane-protein; Ubiquitin Ligase; Quality-control; Heavy-chains; Antigen Presentation; Retro-translocation
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Zeitschrift Nature Communications
Quellenangaben Band: 5, Heft: , Seiten: , Artikelnummer: 3832 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed