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Lou, C.* ; Dallmann, A. ; Geo, R.* ; Brown, T.C.*

Enhanced H-bonding and π-stacking in DNA: A potent duplex-stabilizing and mismatch sensing nucleobase analogue.

Chem. Sci. 5, 3836-3844 (2014)
Verlagsversion DOI
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
X-pyrene is a new nucleic acid duplex stabilizing cytosine analogue that combines enhanced π-stacking, hydrogen bonding and electrostatic interactions to greatly increase the stability of bulged DNA duplexes and DNA/RNA hybrids. X-pyrene is highly selective for guanine as a partner and duplex stability is reduced dramatically when X-pyrene or a neighboring base is mismatched. An NMR study indicates that the pyrene moiety stacks within the helix, and large changes in fluorescence emission on duplex formation are consistent with this. These favorable properties make X-pyrene a promising cytosine analogue for use in a variety of biological applications.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Nucleic-acids; G-clamp; Cytosine Analog; High-affinity; Base Analogs; Oligonucleotides; Pyrene; Rna; Fluorescence; Binding
ISSN (print) / ISBN 2041-6520
e-ISSN 2041-6539
Zeitschrift Chemical Science
Quellenangaben Band: 5, Heft: 10, Seiten: 3836-3844 Artikelnummer: , Supplement: ,
Verlag Royal Society of Chemistry (RSC)
Verlagsort Cambridge
Begutachtungsstatus Peer reviewed