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A review of standardized metabolic phenotyping of animal models.
Mamm. Genome 25, 497-507 (2014)
Metabolic phenotyping of genetically modified animals aims to detect new candidate genes and related metabolic pathways that result in dysfunctional energy balance regulation and predispose for diseases such as obesity or type 2 diabetes mellitus. In this review, we provide a comprehensive overview on the technologies available to monitor energy flux (food uptake, bomb calorimetry of feces and food, and indirect calorimetry) and body composition (qNMR, DXA, and MRI) in animal models for human diseases with a special focus on phenotyping methods established in genetically engineered mice. We use an energy flux model to illustrate the principles of energy allocation, describe methodological aspects how to monitor energy balance, and introduce strategies for data analysis and presentation.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Indirect Calorimetry; Body-composition; Energy-intake; Magnetic-resonance; Insulin-resistance; Adipose-tissue; Mice; Obesity; Mouse; Diet
ISSN (print) / ISBN 0938-8990
Journal Mammalian Genome
Quellenangaben Volume: 25, Issue: 9, Pages: 497-507
Publishing Place New York
Reviewing status Peer reviewed
Institute(s) Institute of Experimental Genetics (IEG)