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Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level.

Analyst 139, 5709-5717 (2014)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Studying cell-to-cell heterogeneity requires techniques which robustly deliver reproducible results with single-cell sensitivity. Through a new fabrication method for the microarrays for mass spectrometry (MAMS) platform, we now have attained robustness and reproducibility in our single-cell level mass spectrometry measurements that allowed us to combine single-cell MAMS-based measurements from different days and samples. By combining multiple measurements, we were able to identify three co-existing phenotypes in an isogenic population of Saccharomyces cerevisiae characterized by distinctively different levels of glycolytic intermediates.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Assisted-laser-desorption/ionization; Mass-spectrometry; Yeast-cells; Metabolomics; Heterogeneity; Matrix; Metabolism; Switch; Flux
Reviewing status