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Generation of targeted mouse mutants by embryo microinjection of TALENs.

Methods 69, 94-101 (2014)
Verlagsversion Postprint DOI
Open Access Green
Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Knockin ; Knockout ; One-cell Embryo ; Gene Targeting ; Talen ; Mouse; Zinc-finger Nucleases; Double-strand Breaks; Homologous Recombination; Dna Cleavage; Effector Nucleases; Mammalian-cells; Gene Disruption; Iii Effectors; Genome; Mice
ISSN (print) / ISBN 1046-2023
e-ISSN 1095-9130
Zeitschrift Methods
Quellenangaben Band: 69, Heft: 1, Seiten: 94-101 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam [u.a.]
Begutachtungsstatus Peer reviewed