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Zolotukhin, A.S.* ; Schneider, R. ; Uranishi, H.* ; Bear, J.* ; Tretyakova, I.* ; Michalowski, D.* ; Smulevitch, S.* ; O'Keeffe, S. ; Pavlakis, G.N.* ; Felber, B.K.*

The RNA transport element RTE is essential for IAP LTR-retrotransposon mobility.

Virology 377, 88-99 (2008)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
We previously identified an RNA transport element (RTE) present at a high copy number in the mouse genome. Here, we show that a related element, RTE-D, is part of a mobile LTR-retrotransposon, which belongs to a family of intracisternal A-particle related elements (IAP). We demonstrate that RTE-D is essential for the mobility of the retrotransposon and it can be substituted by other known RNA export signals. RTE-deficient IAP transcripts are retained in the nucleus, while the RTE-containing transcripts accumulate in the cytoplasm allowing Gag protein expression. RTE-D acts as a posttranscriptional control element in a heterologous reporter mRNA and is activated by the cellular RNA binding protein 15 (RBM15), as reported for the previously described RTE. We identified a complex family of RTE-containing IAPs in mouse and mapped the active RTE-D-containing IAPs to the Mmr10 group of LTR-retrotransposons. These data reveal that, despite a complex evolutionary history, retroelements and retroviruses share the dependency on posttranscriptional regulation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Retrotransposition; Retroelement; mRNA transport; Nuclear export; CTE; Retrovirus; HIV; SRV; NXF1; RBM15
ISSN (print) / ISBN 0042-6822
e-ISSN 0042-6822
Zeitschrift Virology
Quellenangaben Band: 377, Heft: 1, Seiten: 88-99 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed