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Going deeper than microscopy: The optical imaging frontier in biology.
Nat. Methods 7, 603-614 (2010)
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Optical microscopy has been a fundamental tool of biological discovery for more than three centuries, but its in vivo tissue imaging ability has been restricted by light scattering to superficial investigations, even when confocal or multiphoton methods are used. Recent advances in optical and optoacoustic (photoacoustic) imaging now allow imaging at depths and resolutions unprecedented for optical methods. These abilities are increasingly important to understand the dynamic interactions of cellular processes at different systems levels, a major challenge of postgenome biology. This Review discusses promising photonic methods that have the ability to visualize cellular and subcellular components in tissues across different penetration scales. The methods are classified into microscopic, mesoscopic and macroscopic approaches, according to the tissue depth at which they operate. Key characteristics associated with different imaging implementations are described and the potential of these technologies in biological applications is discussed.
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Publikationstyp Artikel: Journalartikel
Schlagwörter Plane illumination microscopy; Raman scattering microscopy; In-vivo detection; Coherence tomography; Photoacoustic microscopy; Projection tomography; High-resolution; Optoacoustic tomography; Multiphoton microscopy; 2-photo microscopy
ISSN (print) / ISBN 1548-7091
Zeitschrift Nature Methods
Quellenangaben Band: 7, Heft: 8, Seiten: 603-614
Verlag Nature Publishing Group
Verlagsort New York, NY