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Arylamines suppress their own activation and that of nitroarenes in V79 Chinese hamster cells by competing for acetyltransferases.

Environ. Health Perspect. 102, 95-97 (1994)
DOI
The effect of 2-aminofluorene (2-AF) on the toxicity of 2-aminoanthracene (2-AA) and 1,6-dinitropyrene (1,6-DNP) was studied in N-acetyltransferase-proficient V79-NHr1A2 cells genetically engineered for the expression of cytochrome P4501A2, and in wild-type V79-NH cells. 2-AA inhibited the growth of V79-NHr1A2 cells and induced the formation of micronuclei at concentrations of 0.1 to 1.0 μM, but was virtually without toxic effects at a concentration of 10 μM. Addition of 2-AF protected against the cytotoxic and genotoxic effects elicited by low concentrations of 2-AA. Half-maximum protection was observed at 0.2 to 0.5 μM 2-AF. The arylamine also prevented the cytotoxicity caused by 1,6-DNP in V79-NH cells and completely suppressed the formation of 1-acetylamino-6-nitropyrene from 1,6-DNP in these cells. The results indicate that arylamines and related N-hydroxyarylamines are substrates for the same acetyltransferase in V79-NH cells. In consequence, arylamines are capable of suppressing the activation of their proximate cytotoxic and genotoxic products in these cells and, presumably, in vivo.
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Publication type Article: Journal article
Document type Scientific Article
Keywords 1,6-dinitropyrene ; 2-aminoanthracene ; 2-aminofluorene ; Acetyltransferase ; Arylamines ; Cytotoxicity ; Genotoxicity ; Micronuclei ; V79-nh Cells ; V79-nhr1a2 Cells
ISSN (print) / ISBN 0091-6765
e-ISSN 1552-9924
Quellenangaben Volume: 102, Issue: SUPPL. 6, Pages: 95-97 Article Number: , Supplement: ,
Publisher Research Triangle Park
Publishing Place NC [u.a.]
Reviewing status Peer reviewed