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Faff, O.* ; Murray, B.A.* ; Erfle, V.F. ; Hehlmann., R.*

Large scale production and purification of human retrovirus-like particles related to the mouse mammary tumor virus.

FEMS Microbiol. Lett. 109, 289-296 (1993)
Verlagsversion DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Human retrovirus-like particles related to mouse mammary tumor virus (MMTV) are secreted in a steroid-dependent manner by the breast cancer cell line T47D. We report the successful large scale production and purification of these particles from culture supernatants of T47D cells and describe the experimental conditions established for this purpose. Thus, mg amounts of particles were produced by large scale culturing of T47D cells in an autoharvesting roller bottle system and purified by differential centrifugation and continuous flow ultracentrifugation on density gradients with a 50% recovery and a 350-fold enrichment.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Human Mmtv-related Particles ; Large Scale Purification
ISSN (print) / ISBN 0378-1097
e-ISSN 1574-6968
Quellenangaben Band: 109, Heft: 2-3, Seiten: 289-296 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed